Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches

On Demand

Dr. Sergi Padilla-Parra

Senior Lecturer, Randall Centre for Cell & Molecular Biophysics, King's College London

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Zhongxiang Jiang

Application Manager, Leica Microsystems

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Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. The gold standard for studying protein-protein interaction is Förster resonance energy transfer (FRET).

Although there are several approaches to demonstrate FRET in biological samples, using fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.

Join Dr. Padilla-Parra for this webinar to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.

After the presentation, join Dr. Roberti for a live showcase demonstration of the STELLARIS 5 confocal system with an integrated WLL, combined with the proprietary Acousto-Optical Beam Splitter (AOBS) and new Power HyD S detectors. Together with the new and unique TauSense technology, STELLARIS 5 sets a new standard for the quality of images and quantity of information generated.

In this webinar, you will learn:

  • how to investigate protein-protein interaction in live samples;
  • ways to get the best out of FRET using fluorescence lifetime information;
  • how non-fitting approaches can be powerful tools for functional imaging;
  • what the minimal fraction of interacting donors (mFD) can tell you about molecular interactions in cells;
  • practical insights into using the STELLARIS 5 confocal system.

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