Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

Ben Steventon

Assistant Professor, Department of Genetics; University of Cambridge

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Ben Steventon obtained his PhD in 2008 in the lab of Roberto Mayor before working as a post-doctoral researcher in numerous labs. He started his research group in 2016 supported by a Wellcome Trust/Royal Society Sir Henry Dale fellowship. In November 2021 he transitioned to an Assistant Professor in the Department of Genetics (University of Cambridge).

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Andrea Boni

Business Excellence Director, Leica Microsystems

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Andrea Boni studied Biotechnology at the San Raffaele University in Milan before moving for his PhD at the EMBL laboratory in Heidelberg (Germany) where he worked on microscopy screening of membrane nuclear protein dynamics. He then transitioned to work on light-sheet development and imaging of intestinal organoids. In 2017 Andrea co-founded Viventis Microscopy, where he was responsible for the imaging applications and sales. After the integration of Viventis Microscopy with Leica Microsystems he joined Leica in May 2024 as Business Excellence Director.

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Explore the complex dynamics of cell fate decisions and morphogenesis through 3D time-lapse microscopy, using real user data to advance our understanding of developmental biology.

In this webinar, you will learn:

• How to use multi-view light sheet microscopy to track cell lineages in vivo during embryonic development
• Ways to use cell tracking data to form hypotheses on gene regulatory networks influencing pattern emergence during morphogenesis
• About the unique versatility of the Viventis Deep microscope in studying the development of various species and embryo models

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time.

Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.

This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.

Join Dr Andrea Boni for a live demonstration of how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.

Follow along with two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.

After this demo, we will introduce the technology behind the Leica Viventis Deep, which combines dual-view illumination and detection in an open-top configuration.

Andrea will demonstrate how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.