Fundamentals of Fluorescence Microscopy
James JonkmanRead Bio
James Created the Advanced Optical Microscopy Facility (AOMF) in 2002 and manages what is now the largest optical microscopy facility in Canada. With 30 instruments at three sites, he and his team help more than 400 users per year with every manner of optical microscopy, from basic fluorescence and brightfield acquisition to advanced live-cell techniques such as FRAP and FRET.Close
What you will learn from this webinar:
- How a fluorescence microscope works;
- What resolution you can expect to achieve from different microscopes;
- How to choose the right fluorophore;
- Four key elements that require optimization for fluoresence microscopy.
Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox. In this presentation I will lay down a foundation for this topic by discussing: How fluorescence microscopes work, choosing fluorophores, and what resolution you can expect to achieve with your microscope. I will end by describing four key elements that each must be optimized to form a good fluorescence microscope.
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