Fundamentals of Fluorescence Microscopy

James Jonkman

What you will learn from this webinar:

  • How a fluorescence microscope works;
  • What resolution you can expect to achieve from different microscopes;
  • How to choose the right fluorophore;
  • Four key elements that require optimization for fluoresence microscopy.


Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox. In this presentation I will lay down a foundation for this topic by discussing: How fluorescence microscopes work, choosing fluorophores, and what resolution you can expect to achieve with your microscope. I will end by describing four key elements that each must be optimized to form a good fluorescence microscope.

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