From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

How correlative light electron microscopy can empower structural biology research

On Demand

Dr. Andreia Pinto

Advanced Workflow Specialist, Leica Microsystems

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Dr. Martin Fritsch

Scientific Officer - Advanced Light Microscopy, EMBL

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Correlative imaging techniques are at the heart of the crusade for better understanding of subcellular biological structures. The use of Correlative Workflows, including Cryo EM, Sub-tomogram averaging, Cryo-FIB, and 3D Volume Imaging, are powerful tools in the field of (in-situ) structural biology, allowing researchers to obtain higher and higher resolution images of complex biological specimens such as organoids and 3D cultures.

In this webinar, experts from the European Molecular Biology Laboratory and Leica Microsystems explore advanced techniques that comprise Correlative Workflows. They cover the full workflow: from the best sample preparation practices, through FIB milling and Electron Microscopy techniques, to image processing and 3D reconstruction.

Key Learnings:

Understanding the techniques required for Correlative Workflows with Cryo FIB and Volume EM, from the workbench to electron beam
Overview of the use of correlative techniques such as Cryo EM, sub-tomogram averaging and 3D Volume Imaging for structural biology research
Application examples of how these techniques have been used to advance our understanding of biological structures

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