Date: Tuesday July 19th, 2022
Time: 3 pm London | 4 pm Berlin | 6 pm Dubai
Randall Centre for Cell & Molecular Biophysics, King's College London
Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. The gold standard for studying protein-protein interaction is Förster resonance energy transfer (FRET).
Although there are several approaches to demonstrate FRET in biological samples, using fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
Join Dr. Padilla-Parra for this webinar to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.
After the presentation, join Dr. Roberti for a live showcase demonstration of the STELLARIS 5 confocal system with an integrated WLL, combined with the proprietary Acousto-Optical Beam Splitter (AOBS) and new Power HyD S detectors. Together with the new and unique TauSense technology, STELLARIS 5 sets a new standard for the quality of images and quantity of information generated.
In this webinar, you will learn: