Visualizing protein-protein interactions by non-fitting and easy FRET-FLIM approaches
LIVE WEBINAR
Date: Tuesday July 19th, 2022
Time: 3 pm London | 4 pm Berlin | 6 pm Dubai
Dr. Sergi Padilla-Parra
Senior Lecturer
Randall Centre for Cell & Molecular Biophysics, King's College London
Dr. Julia Roberti
Product Manager
Leica Microsystems
Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. The gold standard for studying protein-protein interaction is Förster resonance energy transfer (FRET).
Although there are several approaches to demonstrate FRET in biological samples, using fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
Join Dr. Padilla-Parra for this webinar to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.
After the presentation, join Dr. Roberti for a live showcase demonstration of the STELLARIS 5 confocal system with an integrated WLL, combined with the proprietary Acousto-Optical Beam Splitter (AOBS) and new Power HyD S detectors. Together with the new and unique TauSense technology, STELLARIS 5 sets a new standard for the quality of images and quantity of information generated.
In this webinar, you will learn:
- how to investigate protein-protein interaction in live samples;
- ways to get the best out of FRET using fluorescence lifetime information;
- how non-fitting approaches can be powerful tools for functional imaging;
- what the minimal fraction of interacting donors (mFD) can tell you about molecular interactions in cells;
- practical insights into using the STELLARIS 5 confocal system.