See the Hidden: Correlative Techniques for Electron Microscopy

Dr Johnson is head of the Electron Microscopy Facility at the Sir William Dunn School of Pathology at the University of Oxford. Her post-doctoral research focused on plant cell biology at the Umeå Plant Science Centre in Sweden, and she spent several years as an electron microscopist with the Australian Centre for Microscopy and Microanalysis. Dr Johnson has been running the Dunn School EM Facility since 2012, significantly developing its capabilities in biological EM sample preparation, volume EM, cryo-EM, and CLEM. Dr Johnson collaborates on diverse research projects across Oxford, spanning biophysics, structural biology, microbiology, cell biology, biomedicine, and plant biology.

Dr Möbius is the head of the Electron Microscopy Facility at the Max-Planck-Institute for Multidisciplinary Sciences City Campus in Göttingen, Germany. She obtained her Diploma and PhD at the University of Bonn before working as a Postdoc with Hans Geuze and Jan-Willem Slot at the UMC in Utrecht and Gareth Griffith at the EMBL. Her main interest is studying myelin biology with the electron microscope using Tokuyasu EM, high pressure-freezing/freeze substitution, and volume EM using FIB-SEM.

Cristina Capitanio is a PhD candidate in the laboratories of Brenda Schulman and Wolfgang Baumeister at the Max Planck Institute of Biochemistry in Martinsried, Germany. Before becoming interested in cryo-electron tomography, she studied crystallography and molecular biology at the University of Pavia, Italy. Now, her main interest is understanding the ultrastructure of autophagy in different cellular systems, from yeast to human neurons. She also works on developing pipelines that allow cryo-fluorescence signals to target rare cellular events by cryo-FIB milling and cryo-electron tomography.

Anna Bieber is a PhD candidate working in the laboratories of Wolfgang Baumeister and Brenda Schulman at the Max Planck Institute of Biochemistry in Martinsried, Germany. She studied Life Science at the University of Konstanz and worked on in-cell light-induced EPR spectroscopy for her Masters’ thesis. In her current research, she uses correlative cryo-fluorescence microscopy, cryo-focused ion beam (FIB) milling and cryo-electron tomography to reveal the native ultrastructure of autophagy in yeast and mammalian cells. She enjoys constantly developing and improving the technique and applying it directly to gain exciting new insights into the inner workings of the cell.

Dr Fritsch completed his diploma thesis at the Friedrich-Schiller University in Jena. He moved to the Lehrstuhl für Allgemeine und Spezielle Zoologie at the University of Rostock under Prof. Stefan Richter, where he received his Dr. rer. nat in 2012. During his PhD, Martin obtained comprehensive skills in immunohistochemical, histological, and especially confocal microscopy techniques. He focused on the ontogeny of various crustaceans and investigated the development of the nervous system to trace the evolutionary origin of distinct arthropod species. After finishing his PhD, Martin joined the group of Prof. Andreas Wanninger in the department of Integrative Zoology at the University of Vienna, working on mollusc developmental processes. To enrich his theoretical, technical, and methodological profile, he currently works with in-situ hybridization methods to analyze gene expression patterns during ontogeny.

Dr Pinto started her career as a Biomedical Scientist in Histocellular Pathology with a particular interest in electron microscopy. In 2014, she worked at the Primary Ciliary Diagnosis (PCD) in Lisbon. In 2019, Andreia moved to the Royal Brompton Hospital, London, working as a Thoracic Research Associate, where she was responsible for training a deep machine learning platform to recognize patterns in EM images of cilia in diagnosing PCD. She completed her PhD on this topic in 2022 while also investigating new insights into SARS-CoV-2 infection of the respiratory airway. Andreia is currently an Advanced Workflow Application Specialist with Leica Microsystems, based at the Imaging Centre of the European Molecular Biology Laboratory (EMBL) in Heidelberg.

Dr Leroux completed his Master's degree in Biology at the University of Ghent, where he gained experience in biological EM sample preparation. During his PhD at the EMAT research group, University of Antwerp, Frédéric specialized in advanced electron microscopy of composite materials, becoming an EM sample preparation specialist at EMAT. In 2016, he joined Leica Microsystems as Application Specialist Nanotechnology, using his multidisciplinary background and broad microscopy experience to improve EM sample preparation for various materials (polymers, composites, biological and industrial materials).